Journal: bioRxiv
Article Title: Using Imaris to rigorously track PET-defined sites of lung inflammation in Mycobacterium tuberculosis -exposed non-human primates
doi: 10.1101/2025.07.04.663191
Figure Lengend Snippet: We illustrate how PET/CT scans are typically analyzed with Invicro VivoQuant, the standard proprietary packages for PET/CT data analysis ( A ), and with Imaris, proprietary packages typically used for fluorescent microscopy data analysis ( B ). A : With Invicro VivoQuant (i-iv) , the operator analyzes reconstructed 3D images by using 2D slices and manually labeling contours of the lesions, defined by PET signal. Invicro VivoQuant then provides various SUV characteristics, such as max SUV, for ether individual lesions or the whole lung. B : With Imaris (i-vi) , PET/CT DICOM files are imported as 2-channel images, and scans done at different times can be incorporated into the same Imaris file by adding new time frames. Then scans for different time points are aligned using “Correct for drift” routine by using landmarks (6 locations of spine vertebra). Then region of interest ( ROI ) is then identified around each suspected lesion and the data in ROI is processed using 3D routine “Surfaces”. All lesions then can be grouped into the specific group depending on the location of the lesion (e.g., lung vs. lymph nodes).
Article Snippet: One of the main challenges in translatability of the analysis of PET/CT scans from DICOM viewers to Imaris was the lack of a feature or algorithm in Imaris capable of automatically converting channel intensity units to SUV, which is the standard metric used to measure FDG radiotracer absorption in various lung lesions.
Techniques: Positron Emission Tomography-Computed Tomography, Microscopy, Labeling